It’s no longer just a cat’s eyes that glow in the dark! Researchers at the Mayo Clinic have created luminescent, genetically modified cats in an effort to learn more about AIDS.

Feline immunodeficiency virus (FIV) causes feline AIDS, a condition in cats that produces almost identical symptoms to those of AIDS in people infected with human immunodeficiency virus (HIV). Despite the similarity of the viruses, however, it should be noted that people cannot become infected with FIV, and cats cannot be infected by HIV. The similarity of both viruses, however, allows FIV to be used as a model for studying AIDS.
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In this most recent study, the Mayo group inserted two genes into the cats’ eggs prior to fertilization with sperm:

  • An FIV-resistance gene that makes a protein called restriction factor
  • A jellyfish gene to produce GFP, a protein that causes the modified cells to glow green

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Restriction Factor Proteins
Restriction factor proteins usually help the body to block viral infections. But in the case of HIV and FIV infections, these viruses have developed ways to evade attack by the proteins. The rhesus macaque version of restriction factor, however, is able to fight the viruses, so this monkey provided the source of restriction factor.
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Gamete-Targeted Lentiviral Transgenesis
Since cross-breeding of cats and monkeys is not an option, this genetic engineering process was used to introduce the monkey genetic material into cats. Insertion of the monkey restriction factor into the cats was so successful that almost all kittens produced from the genetically modified eggs had the genes for the protective restriction factor protein.

This protein was found all over the body, being tracked by virtue of its green glow, courtesy of the jellyfish gene that had also been introduced at the same time. The GFP protein produced by this gene acts as a marker – it allows the restriction factor proteins to be easily located in the body because it causes them to glow green.

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So What Was The Big Deal About This?
Cells that were subsequently taken out of the cats were found to be resistant to FIV infection when tested.

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The Future?
Although the research is still in its early stages, it certainly shows some exciting potential that could benefit both HIV-infected people, and FIV-infected cats. The Mayo team aims to further study these cats as a means to develop new therapies for people and cats infected with these viruses.

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Photo Credit BBC

Here on the East Coast, we’re awaiting the wrath of Hurricane Irene. Now a category 2 storm, Irene is predicted to travel north along the East Coast throughout the weekend, possibly threatening areas from South Carolina to as far north as here in New England.

If you live in one of these impact zones, now is the time to be considering your evacuation plan, even if there is no immediate evacuation order in your area. And it’s equally important to think about your pets too – if it’s not safe for you to stay home and weather out this potentially destructive storm, it won’t be safe for your pets either.

As a veterinarian, I’ve had plenty of experience over the years helping people to prepare for the worst when it comes to their pets. Here are some tips that might help you prepare to evacuate your pet.

 

 

10 Things To Remember As You Prepare For The Storm

  • Where To Go: Some evacuation shelters don’t allow pets, so contact local animal shelters, pet boarding facilities, and pet-friendly hotels in advance, or at least make a list of their contact details in case you need to relocate your pet.
  • Food Supplies: Have at least a three day supply of your pet’s food ready in appropriate containers for travel. And don’t forget bowls for food and water, and food scoops too.
  • Identity Tags: If your pet has an identity tag, make sure the details are up to date. And if you’ve been thinking about whether to get him one – now is the time to do so!
  • Pet Carrier: This is especially important for smaller dogs and cats. A carrier will not only make it easier for you to transport your pet during an evacuation, but will also ensure that he doesn’t escape. Be sure to clearly identify his carrier with his name and a photograph of him, as well as your contact details.
  • Leash: Don’t forget his leash or harness!
  • Medications: If your pet requires any medications or other health supplies, be sure you have enough to last a couple of weeks, just in case you are unable to get to his veterinarian’s office. Call now for refills if necessary. Some basic first aid supplies can be useful to take along too.
  • Documents: Pack important documents into a zip-lock bag. These may include vaccination records and any other important medical records that you may have for him, as well as a photograph of him, just in case he goes missing and you need to get help searching for him.
  • Litter Box and Litter: If you have a cat, don’t forget his litter box. If possible buy a small one in advance for easier transport. Take at least a three day supply of litter in an airtight container.
  • Familiar Items: Take a couple of your pet’s favorite toys so he has something familiar, and maybe even a small blanket for him. If he requires regular grooming, a small brush will come in handy.
  • Miscellaneous Items: Things such as extra zip-lock bags, baby wipes, and bags for trash can be invaluable extras too.

 

Even though evacuations can be more difficult for people with pets, a little forward planning can make a world of difference. And even if you’re not planning an evacuation, just having these things accessible somewhere can be useful for other eventualities, such as a driving vacation with your pet, or even a house-move. It never hurts to “be prepared”!

 

The recent salmonellosis outbreak linked to ground turkey has spanned at least 31 states so far, reminding us all of the importance of maintaining excellent standards of food hygiene.

But it’s not just our food that’s at risk of infection. In recent years, increasing numbers of pet products have been recalled due to contamination or potential contamination.

In the past couple of years, pet food companies have issued almost three dozen pet food recalls due to salmonella concerns, as well as dog treats such as pigs ears, and treats made from salmon or beef. Just this week, Merrick Pet Care of Amarillo, TX, recalled 248 cases of Doggie Wishbone pet treats due to possible contamination with salmonella. Recently also, Nestlé Purina PetCare recalled some of its Purina One Vibrant Maturity dry cat food when it tested positive for salmonella.

 

 

Although the recent recalls were not associated with illness in people or pets, but rather were a consequence of salmonella detection due to random testing, experts warn that pet foods pose a definite risk of salmonellosis for animals and their owners. And indeed from 2006-2008, 79 people (32 of whom were children under 2 years old) were reported by the Centers for Disease Control and Prevention with salmonella infection associated with contact with dry cat and dog food. Although there were no fatalities, 11 of them were hospitalized.

“The problem of salmonella in pet foods and pet treats, even in pet supplements like vitamins, is something people should be aware of,” reports Dr. Casey Barton Behravesh, a veterinary epidemiologist at the Centers for Disease Control and Prevention.

The problem of contamination of food with salmonella occurs in the factory. In the case of dry food in particular, after processing, kibbles are sent to a room for coating with flavoring. This room provides a moist environment where bacteria can flourish, and salmonella organisms can seemingly persist on the food kibbles for many months.

Symptoms of food poisoning in pets are similar to those in people, and can involve lethargy, anorexia, fever, vomiting and bloody diarrhea for three to five days duration. The most common scenario, however, is the subclinical carrier state, where no signs are seen, but these animals serve as a reservoir of the bacteria.

 

10 TIPS FOR GUARDING AGAINST SALMONELLA INFECTION

A recent report in the Journal of the American Veterinary Medical Association offered recommendations on how owners can reduce the risk of salmonella infection in their pets and family:

  • Wash hands thoroughly before handling food and treats
  • Discourage children, elderly people, and immunosuppressed patient from handling pet food or treats
  • Avoid feeding raw food to pets
  • Avoid buying pet food if it’s container or packaging is damaged
  • Don’t feed anything to your pet that is malodorous or discolored – return it to the store if possible
  • Store pet food products somewhere cool and dry, and according to manufacturer’s guidelines
  • Keep the portion of the food package with the product code and date – in case it needs to be returned due to recall or taint
  • Wash your pet’s bowls and food scoops thoroughly in a sink that you don’t routinely use
  • Avoid feeding your pet in the kitchen
  • If buying pig ears, purchase only in sealed packages, not from open bulk bins

 

And finally, don’t forget to wash your hands after cleaning your cat’s litter box – salmonella organisms can live even after passing through your pet!

 

Kukanich KS (2011): Update on Salmonella spp. contamination of pet food, treats, and nutritional products, and safe feeding recommendations. JAVMA Jun 1; 238(11):1430-1434.

 

 

Fleas are no longer a summertime problem – they can bother your pet all year round, thanks to our cosy, centrally-heated homes that allow flea eggs to incubate and hatch, even in the winter.

I’ve written some articles for the HappyTails Canine Spa, so if you want to control fleas, make it a two-sided attack on the animal as well as his environment.

 

 

There are many varieties of flea control products available for cats and dogs now – your veterinarian can advise you on which is best for your pet (especially if he has developed problems such as secondary pyoderma due to flea allergy dermatitis). But if you have a dog and love trying natural products, check out the online store while you’re over at HappyTails they have some wonderful products!

 

 

 

 

A third of us are infected with Toxoplasma gondii, a parasite that can be transmitted to us by animals. Cats represent a major source of this organism, and are therefore a common route of infection for us since they continually shed the parasite. Consequently we can become infected with Toxoplasma gondii if we come into contact with anything that is contaminated with the organism as a result of a cat’s shedding. Some ways include:

  • Contaminated soil  (when gardening in an area where cats have defecated)
  • Cleaning the cat litter box
  • Contaminated water
  • Undercooked meat (lamb, pork, and venison especially – these animals are infected by cats in the same way as we are)
  • Cooking utensils coming into contact with undercooked, infected meats

Although many people are infected with this parasite, most are unaffected by it, and show no clinical symptoms since the immune system effectively prevents it from causing disease; however, clinical toxoplasmosis can be a real problem for people with weakened immune systems, such as:

  • HIV infected patients
  • Chemotherapy patients
  • Organ-transplant recipients

Additionally, it poses a particular risk for unborn babies. A  woman who comes into contact with the organism for the first time during her pregnancy may transmit it to the fetus in utero, resulting in birth defects or even infant fatalities. Transmission of the parasite to an unborn baby, however, is less likely if the woman has previously come into contact with the organism at least six months or more, prior to becoming pregnant.

Links With Brain Cancer?

Interestingly, a recent study reports a correlation between rates of infection of Toxoplasma gondii, and the incidence of brain cancer. Global data on brain cancer in people from 37 countries was collected and compared with the prevalence of Toxoplasma gondii infection in those regions. The research group reported that brain cancer rates increased in countries where the parasite was more prevalent.

This ecological study, however, merely points to a correlation between the two events – it does not imply that the parasite actually causes brain cancer in people. And certainly the opposite could be true – it’s not impossible that brain cancer could be the driving factor behind Toxoplasma gondii infection.

So as it stands, this report does not prove cause and association, but is predominantly hypothesis-generating, and does provoke scientific curiosity. In the words of one of the authors:“These were the best data available and we felt they were sufficient to take the first step. Working with actual brain cancer patients is an obvious next step, but it would be an expensive proposition.  It is a lot easier to justify the second, expensive step when you have some evidence for the hypothesis. We are hoping that our results motivate others in the field to do further studies.”

Thomas, Lafferty, Brodeur, Elguero, Gauthier-Clerc & Misse. 2011. Incidence of adult brain cancers is higher in countries where the protozoan parasite Toxoplasma gondii is common. Biology Letters

 

Although most kittens learn to use a litter box courtesy of their mother, some may need a helping hand from a new owner – especially those who may have been abandoned or orphaned.

Here are some tips in an article that I wrote for The Pet Store Online.

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With heat warnings and advisories in effect in most states at the moment, and especially spreading east right now, it’s time to take action and ensure that your pets are protected from heat stress.

SOME TIPS TO HELP KEEP YOUR PETS SAFE:
Indoors

  • Provide extra drinking water, especially if your home is not air conditioned
  • Open windows that have screens
  • Close blinds on windows with direct sunlight
  • Leave a fan switched on
Outdoors
  • Never leave a pet in a car unsupervised (even with an outside temperature of just 70 degrees, the temperature inside a car can exceed 150 degrees in minutes)
  • Bring outdoor pets inside if possible
  • Provide a shaded area for them when outside
  • Provide extra drinking water and replace it frequently
  • Secure drinking bowls to prevent them being knocked over
  • Walk away from hot pavements (pets are closer to the ground than we are, so in addition to their paws suffering from the hot pavement, their entire body feels the effect of radiated heat from the ground)

General Tips

  • Keep long coats short in the summer
  • Keep pets indoors as much as possible
  • Reduce your pet’s exercise level
  • Exercise him during the cooler times (early morning, late evening)
Pay Extra Attention To:
  • Overweight pets
  • Pregnant pets
  • Old pets
  • Pets with flat faces (like Persian cats, and dogs such as boxers and pugs)
  • Animals with very short hair
  • Animals with pink skin and white hair
Be On The Lookout For Signs Of Heat Stress:
  • Rapid panting
  • Loud or heavy breathing
  • Increased heart rate
  • Muscle twitching
  • Incoordination
  • Vomiting
What To Do If A Pet Is Suffering From Heat Stress:
  • Cover him with a towel soaked in cool water, or place in bath of cool water if necessary.
  • Seek veterinary attention immediately
  • If you see a pet locked in a car unsupervised, call the authorities immediately – you might just save his life

Heat stroke can be fatal, and animals can succumb to its effects of in less than 15 minutes. The good news, however, is that it is preventable. With a little forward thinking and by following the simple tips above, you can reduce the chances of your pet suffering the potentially fatal consequences of heat stroke.

What are you doing to keep yourself and your pet cool right now?

“Chocolate poisoning” represented one of the “Top 10” pet poisonings reported by the Animal Poison Control Center of the American Society for the Prevention of Cruelty to Animals (ASPCA). This occurs as a result of an adverse reaction to the alkaloid theobromine in chocolate. The toxicity affects dogs more than cats, probably because they  are more likely to consume large quantities of unusual foods chocolate, whereas cats are typically less likely to voluntarily eat chocolate, especially in large quantities. Animals of all ages, however, are affected.

How Much?

According to the ASPCA’s Animal Poison Control Center, mild signs of toxicity are considered to occur in animals after eating around 20 mg of theobromine and caffeine per kg bodyweight, with severe signs arising at 40-50 mg/kg, seizures at 60 mg/kg, with potentially lethal results at 100 mg/kg.

Clinical signs typically occur within 6-12 hours, and how your dog reacts will depend especially on his size, as well as the type of chocolate eaten. Different types of chocolate contain different levels of theobromine: Baking chocolate contains the highest levels, followed by dark chocolate, milk chocolate & white chocolate, respectively.

Some approximate theobromine concentrations in various chocolate products are as follows:

  • Cacao beans: Up to 1200 mg/oz
  • Baking chocolate: 400 mg/oz
  • Dark chocolate: 150 mg/oz
  • Instant cocoa powder: 150 mg/oz
  • Milk chocolate: 50 mg/oz
  • Hot chocolate: 15 mg/oz
  • White chocolate 1 mg/oz

Although these are approximate figures only, they at least provide a sense of the wide range of theobromine concentrations in different products. These figures equate to ingestion of approximately 3 lbs of milk chocolate by a 32 kg Labrador retriever being fatal, while only 7 oz milk chocolate could be fatal to a 4.5 kg Yorkshire terrier.

Clinical Signs

The toxic effects of theobromine affect many organ systems. The first signs of chocolate poisoning, however, tend to be vomiting, diarrhea, nausea, restlessness and increased urination. Signs can ultimately progress to fatal cardiac arrhythmias and seizures.

Treatment

There is no antidote for chocolate poisoning, so treatment tends to be supportive. Since the half life of threobromine is around 18 hours, veterinarians will often induce vomiting in affected dogs within the first 1-2 hours post-ingestion, in particular if the quantity is unknown. Activated charcoal administration can also be useful in reducing the quantity of toxin absorbed. Otherwise, however, treatment is based on the clinical signs of each individual case, with intravenous fluid therapy being commonly used in animals with toxicity-induced dehydration due to vomiting and diarrhea, for instance.

If you do suspect that your pet has eaten an unknown quantity of chocolate, contact your veterinarian immediately for advice. With appropriate, timely treatment, most animals make a complete recovery.

(888) 426-4435 ASPCA Animal Poison Control 

Exposure To Pets In The First Year Of Life May Reduce Risk Of Allergies

The relationship between exposure to pets and allergic disease risk has long been unresolved. Various studies in recent years have examined the effect of early exposure to cats and dogs on allergies in children. The results, however, have been conflicting, with some showing a benefit from having a pet, while others indicated that it may actually make children more prone to developing allergies.

The focus of the study was to evaluate the association between lifetime dog and cat exposure and allergic sensitization to the specific animal at 18 years of age. Researchers collected information from 566 children and their parents about the childrens’ exposure to indoor pets, and their history of allergies.

A study published online ahead of print in the journal “Clinical & Experimental Allergy”, however, suggests that having a pet in the house during the first year of a child’s life could halve the risk of them becoming allergic to animals [“Lifetime Dog and Cat Exposure and Dog- and Cat-Specific Sensitization at Age 18 Years.” Wegienka et al, 2011: 41(7)979-986.]

They followed the children from birth to 18 years old and found that exposure to a cat in the first year of life was associated with a halved risk of having an immune system that was sensitized to cat allergens. The findings for dogs were more complicated, however, and a link between exposure and reduced risk of sensitization later on was found in boys only.

The results of this study now suggest that researchers should further study the first year of life, since this may be the critical window during childhood when indoor exposure to pets may influence sensitization to them.

“This research provides further evidence that experiences in the first year of life are associated with health status later in life, and that early life pet exposure does not put most children at risk of being sensitized to these animals later in life,” said first author Ganesa Wegienka.

Since I’m a veterinary pathologist by training, I thought you might like me to share some aspects of this side of my work with you. 

Some of my daily routine involves microscopic evaluation of sections of tissue from animals, often for diagnostic purposes. So I’ll try and share some interesting cases with you from time to time.

I’ll start off today by sharing information about a case of herpesvirus infection in a cat.

 

History: A two week old kitten died following four days of respiratory distress, with pronounced sneezing and coughing.

Diagnosis: A diagnosis of pneumonia due to FHV-1 (feline herpesvirus-1) infection was made after microscopic evaluation of tissues taken at postmortem examination. 

FHV-1 IN CATS: This is a common feline virus that is transmitted between cats by direct contact and exchange of nasal and ocular fluids. “Feline rhinotracheitis” is the classic condition associated with this virus, and typically manifests as upper respiratory disease and conjunctivitis in kittens, similar to this case. Systemic illness with interstitial pneumonia has also been reported in kittens, and such cases of systemic disease can arise as a result of immunosuppression. Additionally, the virus can cause conjunctivitis as well as facial and nasal dermatitis or stomatitis in adult cats.

The usually clear air spaces in the lung are
filled with inflammatory cells & debris
Higher magnification view showing intense inflammation 
with necrotic tissue and debris, along with the characteristic 
diagnostic finding of viral inclusion bodies in cells. 
The arrow points to a syncitial cell containing multiple nuclei.
These nuclei contain characteristic viral inclusion bodies


Although this is a devastating disease, the good news is that cats can be vaccinated against the causative virus, thus reducing the incidence or severity of disease.

Cats can be carriers of FHV-1 though – in such cases, if a female cat carrying the virus is infected before she is vaccinated, the vaccination will not be effective in eliminating the latent infection. Subsequently, any stress, such as that associated with pregnancy, will result in viral recrudescence and shedding. Following birth, the mother cat will transmit the virus to her kittens in her milk, or through grooming, direct contact or nasal secretions.

So if your cat or kitten has any signs of respiratory disease – be sure to have him or her examined by your veterinarian so as to ensure the correct diagnosis can be made, and appropriate treatment can follow.